Enzymes and Inhibitors
T4 RNA ligase I, 5000 U, G-Biosciences
T4 RNA Ligase I (gp63) catalyzes the ATP-dependent ligation of single-stranded nucleic acids by joining a 5′ phosphate to a 3′ hydroxyl. It is most efficient at ligating the 3′-OH of RNA to the 5′-phosphate of RNA or DNA in single-stranded contexts.
End-Repair dA-tailing Mix, 24 reactions, G-Biosciences
The End-Repair dA-tailing Mix converts damaged or incompatible 5'/3′ ends to 3' A tailed and 5′-phosphorylated DNA.
G-Biosciences T4 UvsX DNA Recombinase
T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes.
G-Biosciences T4 DNA Ligase
T4 DNA Ligase is used for cloning of restriction enzyme generated DNA fragments and PCR products. It is also used for next-generation library preparation, joining linkers and adapters to cohesive or blunt-ended DNA, self-circularization of linear DNA.
Terminal deoxynucleotidyl transferase (TdT), Murine, 2500 U, G-Biosciences
Terminal Transferase catalyzes the untemplated addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
T7 DNA Ligase, 75000 U, G-Biosciences
T7 DNA Ligase is an ATP-dependent enzyme from bacteriophage T7 that catalyzes phosphodiester bond formation between 5′ phosphate and 3′ hydroxyl termini of duplex DNA.
T7 Exonuclease, 2500 U, G-Biosciences
Gene 6 protein of bacteriophage T7 is a dsDNA 5' to 3' exonuclease which also exhibits flap endonuclease activity.
G-Biosciences T4 Polynucleotide Kinase (PNK)
T4 Polynucleotide Kinase (PNK) exhibits 5' polynucleotide kinase and 3' phosphatase activity.
Alkaline Phosphatase, 2500 U, G-Biosciences
TAB5 Alkaline Phosphatase is a heat labile alkaline phosphatase. It catalyzes the dephosphorylation of phosphomonoesters at both 5' and 3' ends of DNA and RNA molecules in a nonspecific manner. Additionally, it eliminates phosphate groups from the 5' and 3' positions of DNA, RNA, and dNTPs.
T4 RNA ligase II, 750 U, G-Biosciences
T4 RNA Ligase II (gp24.1) repairs nicks in dsRNA and can also be used to ligate the 3' terminal OH of RNA to the 5'-terminal phosphate of DNA in a double-stranded structure.
Klenow Exo, 1000 U, G-Biosciences
Klenow fragment (Exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′→5′) and nick-translation (5′→3′) nuclease activities, with a moderate strand displacement activity during DNA synthesis.
