Enzymes and Inhibitors
End-Repair dA-tailing Mix, 24 reactions, G-Biosciences
The End-Repair dA-tailing Mix converts damaged or incompatible 5'/3′ ends to 3' A tailed and 5′-phosphorylated DNA.
T7 DNA Ligase, 75000 U, G-Biosciences
T7 DNA Ligase is an ATP-dependent enzyme from bacteriophage T7 that catalyzes phosphodiester bond formation between 5′ phosphate and 3′ hydroxyl termini of duplex DNA.
T7 Exonuclease, 2500 U, G-Biosciences
Gene 6 protein of bacteriophage T7 is a dsDNA 5' to 3' exonuclease which also exhibits flap endonuclease activity.
Terminal deoxynucleotidyl transferase (TdT), Murine, 2500 U, G-Biosciences
Terminal Transferase catalyzes the untemplated addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
T4 RNA ligase I, 5000 U, G-Biosciences
T4 RNA Ligase I (gp63) catalyzes the ATP-dependent ligation of single-stranded nucleic acids by joining a 5′ phosphate to a 3′ hydroxyl. It is most efficient at ligating the 3′-OH of RNA to the 5′-phosphate of RNA or DNA in single-stranded contexts.
G-Biosciences T4 UvsX DNA Recombinase
T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes.
E. coli Gyrase, 100 U, G-Biosciences
Gyrase is a type IA DNA topoisomerase and it is involved in bacterial DNA supercoiling.
E. coli Exonuclease I, 5000 U, G-Biosciences
Exonuclease I (Exo I), is a highly processive single-stranded DNA-specific exonuclease which removes mono- and dinucleotides from the 3' end of single-stranded DNA molecules (3' to 5' exonuclease activity) leaving double-stranded molecules and the 5' end of single stranded molecules intact.
Thermolabile UDG, 500 U, G-Biosciences
Thermolabile Uracil-DNA Glycosylase (UDG), originating from the species Gadus morhua, removes uracil from DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site.
Exonuclease III, 25000 U, G-Biosciences
Exonuclease III catalyzes the sequential excision of mononucleotides from 3'-hydroxyl termini of duplex DNA, or the RNA strand of a DNA/RNA hybrid.
RNase R, 400 U, G-Biosciences
A C-terminal truncated version of the E.coli RNase R. This truncated enzyme exhibits higher specific activity compared to the full length enzyme.
T3 DNA Ligase, 750000 U, G-Biosciences
T3 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5' phosphate and a 3' hydroxyl termini in duplex DNA; joining blunt and cohesive end termini as well as repairing single stranded nicks in duplex DNA.